EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.44 | A30C/R31I/T32K |
saturation mutagenesis, the mutant shows better kinetics and increased activity for NAD+ compared to the wild-type enzyme |
743817 |
| 1.1.1.44 | A30C/R31I/T32K |
the mutant exhibits a 118.5fold reversal of coenzyme selectivity from NADP+ to NAD+ |
741424 |
| 1.1.1.44 | A30D/R31I/T32I |
saturation mutagenesis, the mutant shows much better kinetics and strongly increased activity for NAD+ compared to the wild-type enzyme |
743817 |
| 1.1.1.44 | A30D/R31I/T32I |
the mutant exhibits a 4278fold reversal of coenzyme selectivity from NADP+ to NAD+ |
741424 |
| 1.1.1.44 | A30E/R31I/T32D |
saturation mutagenesis, the mutant shows better kinetics and increased activity for NAD+ compared to the wild-type enzyme |
743817 |
| 1.1.1.44 | A30E/R31I/T32D |
the mutant exhibits a 1293fold reversal of coenzyme selectivity from NADP+ to NAD+ |
741424 |
| 1.1.1.44 | E131A |
the mutant shows a 2.3fold decrease in turnover number compared to the wild type enzyme |
696246 |
| 1.1.1.44 | H186A |
reduced activity compared to the wild type enzyme |
667707 |
| 1.1.1.44 | H187A |
reduced activity compared to the wild type enzyme |
667707 |
| 1.1.1.44 | K260A |
reduced activity compared to the wild type enzyme |
669487 |
