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Results 1 - 10 of 18 > >>
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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357F217S mutation converts AKR1C33 into a dually coenzyme-specific form and abolishes the activity towards 3-keto-5beta-cholestanes that are substrates specific to AKR1C33, and markedly decreases the sensitivity to 4-pregnenes 739957
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357H216F the mutation decreases 3fold the Km for NADP+. The kinetic constants for bile acids with a 12alpha-hydroxy group are decreased 1.5-7fold and those for the other substrates are increased 1.3-9fold. The mutation decreases the stimulatory effects of the enzyme activity by sulfobromophthalein, clofibric acid and thyroxine, which increases the Km for the coenzyme and substrate of the mutant enzymes more highly than those of the wild type enzyme 724209
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357H216L inactive 724209
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357H216W inactive 724209
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357H216Y the mutation decreases 3fold the Km for NADP+. The kinetic constants for bile acids with a 12alpha-hydroxy group are decreased 1.5-7fold and those for the other substrates are increased 1.3-9fold. The mutation decreases the stimulatory effects of the enzyme activity by sulfobromophthalein, clofibric acid and thyroxine, which increases the Km for the coenzyme and substrate of the mutant enzymes more highly than those of the wild type enzyme 724209
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357K157A site-directed mutagenesis of a catalytic site residue, the NADH binding affinity of K157A mutant is much lower than that of the wild-type resulting in a decreased kcat 760734
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357more when a specific siRNA is used to suppress 3alpha-HSD3 expression without interfering with 3alpha-HSD4, which shares a highly homologous active site, the 5alpha-DHT concentration increases, whereas MCF-7 cell growth is suppressed. Downregulation of 3alpha-HSD3 decreases MCF-7 breast cancer cell growth 740017
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357N272T mutation does not influence cofactor specificity 739957
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357P185A site-directed mutagenesis, analysis of kinetics and structure compared to the wild-type enzyme 743632
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.357P185G site-directed mutagenesis, analysis of kinetics and structure compared to the wild-type enzyme 743632
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