EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.30 | C242S |
PCR derived cDNA clone |
286513 |
| 1.1.1.30 | H141A |
kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 12.9fold lower than wild-type enzyme |
668251 |
| 1.1.1.30 | H141A |
strongly decreased activity |
679792 |
| 1.1.1.30 | H144A |
catalytic efficiency (kcat/Km) is 0.2% of the activity of wild-type HBDH |
698660 |
| 1.1.1.30 | H144A |
the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme |
737914 |
| 1.1.1.30 | H144F |
the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme |
737914 |
| 1.1.1.30 | H144I |
the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme |
737914 |
| 1.1.1.30 | H144L |
the mutant shows activity with levulinic acid |
737912 |
| 1.1.1.30 | H144L |
the mutant shows increased catalytic efficiency with levulinic acid compared to the wild type enzyme |
737914 |
| 1.1.1.30 | H144L/W187A |
the mutant shows reduced catalytic efficiency with levulinic acid compared to the wild type enzyme |
737914 |
