EC Number |
Protein Variants |
Reference |
---|
1.1.1.274 | more |
construction of enzyme gene knockout mutant M-AKR, that shows decreased degradation activity with testosterone, estradiol, oestrone, and methyltestosterone compared to the wild-type enzyme. Compared to the wild-type, the mutation of the endogenous 2,5DKR gene results in lower degradation of estradiol and methyltestosterone but has no effct on degradation of estrone and testosterone |
-, 742259 |
1.1.1.274 | more |
evaluation of the food grade expression systems NICE, Lactococcus lactis, and pSIP, Lactobacillus plantarum, for the production of 2,5-diketo-D-gluconic acid reductase from Corynebacterium glutamicum that also satisfies food safety requirements. Both systems are suitable for 2,5-DKG reductase expression, maximum production yields are obtained with Lactobacillus plantarum/pSIP609 by pH control at 6.5, overview |
723915 |
1.1.1.274 | F22Y/A272G |
increased activity compared to the wild-type enzyme, substrate inhibition at substrate concentrations above 17.5 mM |
657308 |
1.1.1.274 | Q192R |
isoenzyme A, 2.5fold increase in kcat |
440307 |
1.1.1.274 | F22Y/K232G/R235T/R238E/A272G |
isoenzyme A, 24fold increase in kcat for NADH |
440311 |
1.1.1.274 | K232 |
isoenzyme A, designed to improve the ability to use NADH as cofactor |
440310 |
1.1.1.274 | K232Q |
isoenzyme A, designed to improve the ability to use NADH as cofactor |
440310 |
1.1.1.274 | K232S |
isoenzyme A, designed to improve the ability to use NADH as cofactor |
440310 |
1.1.1.274 | R235G |
isoenzyme A, designed to improve the ability to use NADH as cofactor |
440310 |
1.1.1.274 | R235T |
isoenzyme A, designed to improve the ability to use NADH as cofactor |
440310 |