EC Number |
Protein Variants |
Reference |
---|
1.1.1.274 | A272G |
mutation increases Km and kcat compared to the wild-type enzyme |
657308 |
1.1.1.274 | F22Y |
isoenzyme A, similar kcat as wild-type |
440307 |
1.1.1.274 | F22Y |
mutation reduces Km and increases kcat by 50% compared to the wild-type enzyme |
657308 |
1.1.1.274 | F22Y/A272G |
increased activity compared to the wild-type enzyme, substrate inhibition at substrate concentrations above 17.5 mM |
657308 |
1.1.1.274 | F22Y/A272G |
isoenzyme A, reduced kcat for NADH |
440311 |
1.1.1.274 | F22Y/K232G/R235G/R238E/A272G |
isoenzyme A, increase in kcat for NADH |
440311 |
1.1.1.274 | F22Y/K232G/R235G/R238H/A272G |
isoenzyme A, increase in kcat for NADH |
440311 |
1.1.1.274 | F22Y/K232G/R235T/R238E/A272G |
isoenzyme A, 24fold increase in kcat for NADH |
440311 |
1.1.1.274 | F22Y/K232G/R238H/A272G |
isoenzyme A, increase in kcat forNADH |
440311 |
1.1.1.274 | F22Y/K232G/R238H/A272G |
mutant shows a higher activity with NADH compared to the wild-type enzyme |
657308 |