EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.247 | A25V |
site-directed mutagenesis, the mutant shows a marginal increase in neopine formation compared to wild-type |
763579 |
| 1.1.1.247 | A25V/E111D/T145S/S165C |
site-directed mutagenesis, the mutant shows a reduction in neopine formation compared to wild-type |
763579 |
| 1.1.1.247 | E111D/T145S/S165C |
site-directed mutagenesis, the mutant shows a reduction in neopine formation compared to wild-type |
763579 |
| 1.1.1.247 | F129L |
site-directed mutagenesis, the mutant of COR-B shows unaltered neopine formation compared to wild-type |
763579 |
| 1.1.1.247 | K41N |
site-directed mutagenesis, the mutant of COR-B shows unaltered neopine formation compared to wild-type |
763579 |
| 1.1.1.247 | L129F |
site-directed mutagenesis, the mutant shows a higher neopine yield compared to wild-type |
763579 |
| 1.1.1.247 | more |
combinations and permutations of the three substitutions N41K, L129F and S279W do not produce a synergistic effect |
763579 |
| 1.1.1.247 | more |
recombinant coexpression of isozyme COR-B with thebaine 6-O-demethylase (T6ODM) in Saccharomyces cerevisiae strain CEN.PK fed with thebaine, the strain expressing T6ODM and COR-B accumulates approximately 2fold more codeine and 10fold more neopine compared to wild-type |
763579 |
| 1.1.1.247 | more |
recombinant coexpression of isozyme COR1.3 with thebaine 6-O-demethylase (T6ODM) in Saccharomyces cerevisiae strain CEN.PK fed with thebaine. In the recombinant strain, codeine and neopine accumulate to similar levels |
763579 |
| 1.1.1.247 | more |
the COR1.4 single-substitution mutant A (A25V) results in a marginal increase in neopine formation, whereas the triple C (E111D), E (T145S) and F (S165C), and the quadruple A (A25V), C (E111D), E (T145S) and F (S165C), mutants result in higher reductions in neopine levels |
763579 |
