EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.244 | A164F |
mutation improves the specific activity of the enzyme toward methanol compared to that of the wild-type enzyme. Mutant shows a slightly higher turnover rate than that of wild-type, although the KM value is increased compared to that of wild-type |
-, 763060 |
| 1.1.1.244 | A164P |
3fold increase in kcat/Km as compared to wild-type enzyme |
-, 762559 |
| 1.1.1.244 | A164P |
4.7fold increase in kcat/Km as compared to wild-type enzyme |
-, 762559 |
| 1.1.1.244 | A169C |
site-directed mutagenesis |
741715 |
| 1.1.1.244 | A169I |
site-directed mutagenesis |
741715 |
| 1.1.1.244 | A169L |
site-directed mutagenesis |
-, 741715 |
| 1.1.1.244 | A169M |
site-directed mutagenesis |
741715 |
| 1.1.1.244 | A169P |
site-directed mutagenesis |
741715 |
| 1.1.1.244 | A169V |
from library screening, mutant CT1-2, mutant variants CT1-2, CT2-1, and CT4-1 show 5 to 10fold reduced specific activity towards ethanol and 6 to 8fold reduced for propanol compared to wild-type |
-, 741715 |
| 1.1.1.244 | A26V |
site-directed mutagenesis, mutant CT2-2, the mutation A26V alone demolishes Mdh activity, inactive mutant |
-, 741715 |
