EC Number   |
Protein Variants |
Reference |
|---|
   1.1.1.237 | more |
cultures fed with precursors, namely L-Phe, L-Tyr, and cucumber juice, at different concentrations show Phe an 1.5, 2.1, and 1.6fold increase in rosmarinic acid accumulation within 48-72 h, respectively. In this study, we focused on the function of individual precursor on key enzymes involved in RA biosynthesis. The phenylalanine ammonia lyase activity was significantly upregulated after Phe feeding, while tyrosine aminotransferase and hydroxyphenylpyruvate reductase activities are improved with Tyr treatment. Rosmarinic acid synthase activity is significantly enhanced by all three precursors, overview |
741057 |
| 1.1.1.237 | more |
expression analysis of HPPR2, HPPR3, and other pathway-related enzymes in mutant strains, HPPR3 mutant phenotype, overview |
-, 761180 |
| 1.1.1.237 | more |
hairy root lines of Coleus blumei carrying HPPR or rosmarinic acid synthase (RAS) RNAi suppression and overexpression constructs are established using Agrobacterium rhizogenes-mediated transformation. Hairy root lines showing undetectable HPPR or RAS mRNA levels displayed strongly reduced rosmarinic acid contents with reduction of up to 92% compared to control hairy root lines. An increase of rosmarinic acid levels up to 176% occurs in overexpression hairy root lines with equally increased HPPR transcript levels while RAS overexpression results in co-suppression effects with reduced rosmarinic acid levels |
741143 |
| 1.1.1.237 | more |
overexpression of HPPR does not lead to the enhancement of phenolic acid production in comparison with control. Single HPPR transgenic lines and TAT-HPPR co-transgenic lines present much higher rosmarinic acid and lithospermic acid B accumulation. Although possessing a relatively low TAT and HPPR transcript level compared with the single-gene transgenic lines, the TAT-HPPR co-transgenic lines produce over 16.1times rosmarinic acid and 18.8times lithospermic acid B than that in control lines. TAT-HPPR co-transgenic lines possess the lowest accumulation level of homogentisic acid, a compound involved in the competitive branch of rosmarinic acid biosynthesis and produced by HPPD from 4-hydroxyphenylpyruvic acid precursor. This most likely suggested a metabolic flux shift from the homogentisic acid branch to the rosmarinic acid pathway |
739871 |
