EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.2 | A18P/K40N/Q165H/D182H/E202G/R219M |
mutant with increased specific activity towards 2,5-hexanedione compared to the wild type enzyme |
697833 |
| 1.1.1.2 | D194A |
mutation in putative metal-coordinating residue, almost complete loss of activity |
-, 737536 |
| 1.1.1.2 | D275P |
mutation significantly enhances the thermal stability of EhADH1 |
689955 |
| 1.1.1.2 | F197W |
site-directed mutagenesis, kinetics compared to the wild-type enzyme |
760278 |
| 1.1.1.2 | G211C |
site-directed mutagenesis, cofactor binding and kinetic analysis compared to the wild-type enzyme |
746361 |
| 1.1.1.2 | G211S |
site-directed mutagenesis, cofactor binding and kinetic analysis compared to the wild-type enzyme |
746361 |
| 1.1.1.2 | G37D |
regular PCR with site-specific mutation for both DNA strands, the gene is divided into two separate amplification products, preference for NAD+ rather than NADP+, depends on an a interaction between the adenosine ribose moiety of NAD+ and the inserted aspartate side chain |
675348 |
| 1.1.1.2 | H198A |
mutation in putative metal-coordinating residue, almost complete loss of activity |
-, 737536 |
| 1.1.1.2 | H267A |
mutation in putative metal-coordinating residue, almost complete loss of activity |
-, 737536 |
| 1.1.1.2 | H281A |
mutation in putative metal-coordinating residue, almost complete loss of activity |
-, 737536 |
