EC Number   |
Protein Variants  |
Reference |
|---|
    6.3.4.2 | A20R |
mutation disrupts cytoophidium assembly |
744847 |
| 6.3.4.2 | C379A |
mutant enzyme is fully active with ammonia but has no glutamine-dependent activity, no inhibition by glutamate gamma-semialdehyde |
648963 |
| 6.3.4.2 | C404G |
inactive |
-, 745736 |
| 6.3.4.2 | D107A |
enzyme exhibits wild-type NH3-dependent activity and affinity for glutamine, but impaired glutamine-dependent CTP formation, affinity of the mutant enzyme for GTP is reduced 2-4fold |
648962 |
| 6.3.4.2 | D149E |
the single point mutation in the resistant strain L2/CPEC results in loss of CTP feedback inhibition, cells are resistant to the cytotoxic effects of cyclopentenyl cytosine |
-, 648995 |
| 6.3.4.2 | D70A |
mutation in the ATP binding site, mutant exhibits approximately twofold increase in the number of cells containing filaments |
-, 745736 |
| 6.3.4.2 | DELTA20 |
deletion of the N-terminal 20 amino acids alone is sufficient to disrupt cytoophidium assembly, mutant protein forms distinct cytoplasmic structures which appear as large clusters |
744847 |
| 6.3.4.2 | E103A |
mutant enzyme exhibits no glutamine-dependent activity and is only partially active with NH3 |
648962 |
| 6.3.4.2 | E146A |
mutation in the ATP binding site, mutant exhibits approximately twofold increase in the number of cells containing filaments |
-, 745736 |
| 6.3.4.2 | E161K |
mutation in CTP binding site, filament formation is completely disrupted and the enzyme can only form foci. Mutation decreases the affinity of the enzyme for CTP |
-, 745736 |
