EC Number   |
Protein Variants |
Reference  |
|---|
    5.1.3.37 | D152G |
mutation eliminates almost all of both the lyase and epimerase activities |
652156 |
| 5.1.3.37 | more |
a truncated form of isoform AlgE1 (AlgE1-1) is converted to a combined epimerase and lyase by replacing the 5'-798 base pairs in the algE1-1 gene with the corresponding A-module-encoding DNA sequence from algE7 |
652156 |
| 5.1.3.37 | more |
a truncated form of isoform AlgE1 (AlgE1-1) is converted to a combined epimerase and lyase by replacing the 5'-798 base pairs in the algE1-1 gene with the corresponding A-module-encoding DNA sequence from bifunctional isoform algE7 |
652156 |
| 5.1.3.37 | more |
construction of mutant enzymes that introduce a high level of G-blocks in poly(beta-(1->4)-D-mannuronate) more efficiently than the wild-type enzymes from Azotobacter vinelandii when employed for in vitro epimerization reactions. Shuffling of the genes encoding isoforms AlgE1 to AlgE6 leads to two epimerases that are more efficient in introducing G-blocks in poly(beta-(1->4)-D-mannuronate) than the naturally occurring enzymes, and one of these acts kinetically different than the G-block former AlgE6 |
733461 |
| 5.1.3.37 | more |
construction of a variety of truncated forms of isoform AlgE1. An A module alone is sufficient for epimerization and module A1 catalyzes the formation of contiguous stretches of G residues in the polymer, while module A2 introduces single G residues. The epimerization reaction is Ca2+ dependent, and both the A and R modules bind this cation. The R modules appear to reduce the Ca2+ concentration needed for full activity and also stimulate the reaction rate when positioned both N- and C-terminally |
734060 |
| 5.1.3.37 | more |
division of algE1 into two parts based on the modular type of structure, and expression of each part in Escherichia coli. AlgE1 contains two catalytic domains, AlgE1-1, which introduces both G-blocks and MG-blocks, and AlgE1-2, which only introduces MG-blocks. AlgE1-1 has a much lower specific activity than both AlgE1-2 and AlgE1 |
734128 |
| 5.1.3.37 | D173E |
54% residual activity |
734159 |
| 5.1.3.37 | D178E |
complete loss of activity |
734159 |
| 5.1.3.37 | D178N |
complete loss of activity |
734159 |
| 5.1.3.37 | F122Y |
65% residual activity |
734159 |
