EC Number   |
Protein Variants |
Reference |
|---|
    4.4.1.15 | E295S |
By site-directed mutagenesis. The Pseudomonas putida UW4 single mutant is constructed using pET30a (+) with the full-length ACC deaminase as the template |
-, 694780 |
| 4.4.1.15 | E295S/L322T |
The double mutant is constructed using the E295S mutant as the template. |
-, 694780 |
| 4.4.1.15 | H80Q |
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme |
730698 |
| 4.4.1.15 | Q77H |
site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 30% activity with beta-chloro-D-alanine |
730698 |
| 4.4.1.15 | S358E |
By site-directed mutagenesis. The single mutants of the tomato enzyme are constructed using pET30 Xa/LIC with the full-length putative ACC deaminase as the template |
694780 |
| 4.4.1.15 | S358E/T386L |
The double mutant is constructed using an additional round of mutagenesis and with the S358E single mutant as the template |
694780 |
| 4.4.1.15 | S78A |
site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 20% activity with beta-chloro-D-alanine |
730698 |
| 4.4.1.15 | T288E |
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme |
730698 |
| 4.4.1.15 | T315L/T288E |
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme |
730698 |
| 4.4.1.15 | Y261F |
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme |
730698 |
