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Results 1 - 10 of 11 > >>
EC Number
Amino acid exchange
Commentary
Reference
E295S
By site-directed mutagenesis. The Pseudomonas putida UW4 single mutant is constructed using pET30a (+) with the full-length ACC deaminase as the template
E295S/L322T
The double mutant is constructed using the E295S mutant as the template.
H80Q
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme
Q77H
site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 30% activity with beta-chloro-D-alanine
S358E
By site-directed mutagenesis. The single mutants of the tomato enzyme are constructed using pET30 Xa/LIC with the full-length putative ACC deaminase as the template
S358E/T386L
The double mutant is constructed using an additional round of mutagenesis and with the S358E single mutant as the template
S78A
site-directed mutagenesis, the mutant is inactive with respect to D-Cys, but retains significant 20% activity with beta-chloro-D-alanine
T288E
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme
T315L/T288E
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme
Y261F
site-directed mutagenesis, the mutant shows altered substrate specificity and activity compared to the wild-type enzyme
Results 1 - 10 of 11 > >>