EC Number   |
Protein Variants  |
Reference |
|---|
    4.3.1.2 | C361A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 47% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | C361K |
mutant shows no reactivity. Conversion 2h: 10% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | F170A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 63% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | H194A |
enhanced diastereoselectivities |
703197 |
| 4.3.1.2 | K331A |
enhanced diastereoselectivities |
703197 |
| 4.3.1.2 | L384A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 61% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | L384A |
mutant displays pronounced amination activity towards 2-hexylfumarate ad decreased amination activity towards mesaconate compared to wild-type. Mutant displays a broad electrophile scope including fumarate derivatives with alkyl, aryl, alkoxy, aryloxy, alkylthio and arylthio substituents at the C2 position |
730442 |
| 4.3.1.2 | Q172A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 16% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | Q172N |
mutant shows no reactivity. Conversion 2h: 6% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
| 4.3.1.2 | Q329A |
enhanced diastereoselectivities |
703197 |
