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Results 1 - 3 of 3
EC Number
Amino acid exchange
Commentary
Reference
DLCR6 (cimA::Gm)
citramalate synthase knockout mutant
DLCR7 (tdcB::Kn cimA::Gm)
double (ammonia-lyase (EC 4.3.1.19) and citramalate synthase) knockout mutant. DLCR5 (tdcB::Kn) a threonine ammonia-lyase knockout mutant (EC 4.3.1.19) is also constructed
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strain DL1 used to construct strains. Construction of mutants via single-step gene replacement. Mutants lacking both enzymes (citramalate synthase and threonine ammonia-lyase) are auxotrophs for isoleucine, whereas single mutants are capable of growth in the absence of isoleucine
Results 1 - 3 of 3