EC Number |
Protein Variants |
Reference |
---|
3.6.5.4 | more |
4.5S RNA tetraloop mutants UUCG, UUUU, CUUC, GUAA and GAAA |
690025 |
3.6.5.4 | more |
binding kinetics and competing inhibitions with several bacterial cpFtsY mutants, affinities of mutant bacterial cpFtsYs for cpSRP54, and complex formations, overview |
718911 |
3.6.5.4 | S220A |
bypass requirement for GEF |
644176 |
3.6.5.4 | more |
construction of a bi-cistronic construct containing human SRalpha subunit and murine SRbeta subunit lacking the transmembrane domain and cloning in the pET16b vector. Linker deletion variants of SR are created using the same construct |
757758 |
3.6.5.4 | more |
construction of several deletion mutants: SRbeta-DELTATM, SRbetaDELTA4, SRbetaDELTA5, SRbeta1C1-DELTATM, SRbeta-DELTAloop, and SRbeta-loop2, phenotypes, altered SRalpha binding of the truncated mutants, overview. Deletion of the last 6 amino-acids from the carboxyl-terminus of the SRbeta GTPase domain results in a molecule (SRbetaC1) with primarily type I topology, similar to SRbeta |
757430 |
3.6.5.4 | more |
construction of the FtsYDELTAN1 mutant, and creation of a fusion construct comprising two FtsYDELTAN1 molecules linked via a 31-amino-acid GS linker, similar to the situation for the FtsY/Ffh heterodimer (FtsYDELTAN12) |
758489 |
3.6.5.4 | Thr112Ala |
deficient in GTP hydrolysis |
-, 644178 |
3.6.5.4 | G83A |
deletion or substitution of G83 by any other nucleotide completely abolishes the stimulatory effect of the SRP RNA on GTP hydrolysis. Despite defective GTP hydrolysis, the G83A mutant shows any detectable defect in the efficiency of GTPase docking at the distal end |
734245 |
3.6.5.4 | D329A |
diminished GTPase activity |
644174 |
3.6.5.4 | T326N |
diminished GTPase activity |
644174 |