EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.55 | C303A |
catalytically inactive |
665588 |
| 3.4.22.55 | C303A |
inactive |
664882 |
| 3.4.22.55 | C320A |
catalytic residue, inactive mutant enzyme |
713154 |
| 3.4.22.55 | C320A |
catalytically inactive |
732048 |
| 3.4.22.55 | C320A |
forms a dimer only when cell are treated with DRB |
664753 |
| 3.4.22.55 | D152A |
pro-caspase mutant, like the wild-type, this mutant is efficiently processed between the large and the small subunit, however, it is not further processed to seperate the prodomain from the large subunit |
664882 |
| 3.4.22.55 | D316A |
fusion of the linker to the large subunit, toxic when expressed in yeast |
664882 |
| 3.4.22.55 | D316A/D330A |
abolishes auto-processing and reduces enzymatic activity dramatically, 840fold decrease in activity |
664882 |
| 3.4.22.55 | D316G |
uncleavable |
665588 |
| 3.4.22.55 | D330A |
fusion of the linker to the small subunit, slightly greater deleterious effect on enzyme activity than fusion to the large subunit (D316A), toxic when expressed in yeast |
664882 |
