EC Number |
Protein Variants |
Reference |
---|
3.4.22.53 | E504S |
Ca2+ concentration required for half-maximal activity is 0.129 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme |
644019 |
3.4.22.53 | K234W |
Ca2+ concentration required for half-maximal activity is 0.159 mM compared to 0.242 mM for the wild-type enzyme |
644019 |
3.4.22.53 | K234S |
Ca2+ concentration required for half-maximal activity is 0.183 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme |
644019 |
3.4.22.53 | K226S |
Ca2+ concentration required for half-maximal activity is 0.226 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme |
644019 |
3.4.22.53 | K230E |
Ca2+ concentration required for half-maximal activity is 0.256 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme |
644019 |
3.4.22.53 | K230S |
Ca2+ concentration required for half-maximal activity is 0.261 mM compared to 0.242 mM for the wild-type enzyme |
644019 |
3.4.22.53 | C105S |
inactive mutant enzyme |
644016 |
3.4.22.53 | H262A |
inactive mutant enzyme |
644016 |
3.4.22.53 | N286A |
inactive mutant enzyme |
644016 |
3.4.22.53 | S369D |
inactive mutant enzyme |
644047 |