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Results 1 - 10 of 30 > >>
EC Number
Amino acid exchange
Commentary
Reference
C105S
inactive mutant enzyme
C105S
inactive mutant enzyme. The mutant enzyme provides a purified calpain, that is stable to autolysis and oxidation, which is likely to facilitate crystallization in both the presence and absence of calcium
C105S
mutant enzyme of mutant large subunit m-C105S-80K, coexpressed with 30000 Da subunit in Sf-9 cells does not degrade casein nor the artificial substrate succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide. The mutant enzyme does not show autolytic activity with Ca2+
D346E
mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation
D362K
mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation
E504S
Ca2+ concentration required for half-maximal activity is 0.129 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme; mutation decreases specific activity to 90% compared to wild-type enzyme
G423R
mutant with decreased enzymatic activity, increased rate of autoproteolytic degradation
H262A
inactive mutant enzyme
K225S
mutation decreases specific activity to 88% compared to wild-type enzyme
K226S
Ca2+ concentration required for half-maximal activity is 0.226 mM compared to 0.242 mM for the wild-type enzyme. Refined structure of the mutant enzyme in absence of Ca2+ is indistinguishable from wild-type enzyme
Results 1 - 10 of 30 > >>