EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.49 | C2029A |
- |
638869 |
| 3.4.22.49 | S1126A |
a doxycycline-induced overexpression of S1126A mutant in HEK-293 cells leads to an increase of the G2/M cell population from 24 to 49% within 24 h compared to wild-type. Giemsa-stained prometaphase-chromosomes from S1126A expressing cells are mostly separated (61%), whereas those from wild-type or T1346E/T1363E/S1399D expressing cells are almost always paired (78 and 83%, respectively) |
680926 |
| 3.4.22.49 | S1121A |
a mouse strain is created bearing a S1121A mutation: S1121A mutation causes infertility in mice, germ cells in the mutants are depleted during development. S1121A causes chromosome misalignment during proliferation of the postmigratory primordial germ cells, resulting in mitotic arrest, aneuploidy, and eventual cell death |
682481 |
| 3.4.22.49 | S1126A/T1346A |
active mutant |
709220 |
| 3.4.22.49 | H1531A |
active site point mutation prevents Scc1 from being cleaved after binding |
638864 |
| 3.4.22.49 | more |
analysis of the homozygous zebrafish mutant cds (cease&desist), bearing a mutation in the separase gene, reveals high levels of polyploidy and aneuploidy, spindle defects, and a mitotic exit delay in mutant embryos. Carcinogenesis studies demonstrate that cds heterozygous adults have an eightfold increase in the percentage of fish bearing epithelial tumors |
680013 |
| 3.4.22.49 | E1483R/R1486E |
autoproteolytically cleaved like wild-type |
638869 |
| 3.4.22.49 | E1503R/R1506E |
autoproteolytically cleaved like wild-type |
638869 |
| 3.4.22.49 | E1532R/R1535E |
autoproteolytically cleaved like wild-type |
638869 |
| 3.4.22.49 | T1346E/T1363E/S1399D |
changing the phosphorylation sites within the Cdc6-like domain to acidic residues results in a separase that can still be inhibited by Cdk1 in vivo |
680926 |
