EC Number |
Protein Variants |
Reference |
---|
3.4.22.44 | C151A |
catalytically inactive mutant TVMV protease |
718366 |
3.4.22.44 | C151A |
site-directed mutagenesis, a NIa-Pro active site mutant |
718449 |
3.4.22.44 | E189L |
no self-cleavage |
137282 |
3.4.22.44 | E191A |
site-directed mutagenesis, a VPg-Pro mutant |
718449 |
3.4.22.44 | G224R |
almost no self-cleavage of the 25 kDa protein |
-, 137276 |
3.4.22.44 | K65A/K67A/C151A |
catalytically inactive mutant TVMV protease |
718366 |
3.4.22.44 | more |
a truncation mutant of TVMV protease lacking the 20 C-terminal amino acid residues, TVMV1-217 protease, shows reduced activity compared to the wild-type enzyme |
718366 |
3.4.22.44 | more |
comparison of the catalytic activities of wild-type, mutant and C-terminal truncated proteases |
-, 137276 |
3.4.22.44 | more |
overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol |
718264 |
3.4.22.44 | S129A |
site-directed mutagenesis, a NIa-Pro phosphorylation-deficient mutant |
718449 |