Refine search

Search Engineering

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 12 > >>
EC Number
Amino acid exchange
Commentary
Reference
C151A
catalytically inactive mutant TVMV protease
C151A
site-directed mutagenesis, a NIa-Pro active site mutant
E189L
no self-cleavage
E191A
site-directed mutagenesis, a VPg-Pro mutant
G224R
almost no self-cleavage of the 25 kDa protein
K65A/K67A/C151A
catalytically inactive mutant TVMV protease
more
a truncation mutant of TVMV protease lacking the 20 C-terminal amino acid residues, TVMV1-217 protease, shows reduced activity compared to the wild-type enzyme
more
comparison of the catalytic activities of wild-type, mutant and C-terminal truncated proteases
more
overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol
S129A
site-directed mutagenesis, a NIa-Pro phosphorylation-deficient mutant
Results 1 - 10 of 12 > >>