EC Number |
Protein Variants |
Reference |
---|
3.2.2.8 | H239A |
dramatic increase in Km for uridine, unchanged kcat |
657373 |
3.2.2.8 | H82N |
small increase in Km, increase in kcat |
657373 |
3.2.2.8 | Q227A |
the mutation causes an increase of kcat for uridine and inosine |
696258 |
3.2.2.8 | Q227F |
the mutation causes an increase of kcat for uridine and inosine |
696258 |
3.2.2.8 | Q227Y |
the mutation has a strong, enhancing effect on the hydrolysis of inosine, and the catalytic efficiency for the purinic substrate is increased by a factor of 7.6 |
696258 |
3.2.2.8 | T223A |
the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine |
696258 |
3.2.2.8 | T223F |
the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine |
696258 |
3.2.2.8 | T223F/Q227Y |
the mutant shows a 2fold increase in catalytic efficiency toward inosine |
696258 |
3.2.2.8 | T223Y |
the mutation does not affect the specificity of the enzyme toward inosine or uridine |
696258 |
3.2.2.8 | T223Y/Q227Y |
the mutant displays a catalytic efficiency toward inosine that is more than 50fold increased compared to that of wild type enzyme |
696258 |