EC Number |
Protein Variants |
Reference |
---|
3.2.2.8 | T223Y/Q227Y |
contrary to wild-type, mutant is able to process inosine |
751203 |
3.2.2.8 | H239A |
dramatic increase in Km for uridine, unchanged kcat |
657373 |
3.2.2.8 | more |
mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination |
700741 |
3.2.2.8 | H82N |
small increase in Km, increase in kcat |
657373 |
3.2.2.8 | T223Y/Q227Y |
the mutant displays a catalytic efficiency toward inosine that is more than 50fold increased compared to that of wild type enzyme |
696258 |
3.2.2.8 | T223F/Q227Y |
the mutant shows a 2fold increase in catalytic efficiency toward inosine |
696258 |
3.2.2.8 | Q227A |
the mutation causes an increase of kcat for uridine and inosine |
696258 |
3.2.2.8 | Q227F |
the mutation causes an increase of kcat for uridine and inosine |
696258 |
3.2.2.8 | T223Y |
the mutation does not affect the specificity of the enzyme toward inosine or uridine |
696258 |
3.2.2.8 | T223A |
the mutation does not improve significantly the catalytic efficiency of YeiK toward inosine |
696258 |