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Results 1 - 10 of 26 > >>
EC Number
Amino acid exchange
Commentary
Reference
D101G
in vitro activity relative to wild-type: 71%, monomer/dimer ratio: 34/66
D83G
in vitro activity relative to wild-type: 100%, monomer/dimer ratio: 82/18
E18D
in vitro activity relative to wild-type: 56%, monomer/dimer ratio: 40/60
G153D
crystal structure of mutant is determined at 2.8 A: The protein crystallizes in the hexagonal space group P31 with 4 chains in the asymmetric unit. Mutant shows reduced activity and is composed of a monomer. Results from isothermal titration calorimetry experiments show that MjCobYG153D has 10fold higher affinity for GTP than wild-type, but fails to bind the corrinoid substrate; in vitro activity relative to wild-type: 2%, monomer/dimer ratio: 54/46
G153D
residues R13 and K19 of the mutant directly coordinate the phosphate group of GTP in the X-ray structure. The X-ray structure of the CobYG153D:GTP complex is modeled as a homodimer
G8D
mutant non-functional, monomer/dimer ratio: 33/67
H45A
site-directed mutagenesis
H45A/H46A
site-directed mutagenesis
H46A
site-directed mutagenesis
H46N
site-directed mutagenesis
Results 1 - 10 of 26 > >>