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Results 1 - 10 of 17 > >>
EC Number
Amino acid exchange
Commentary
Reference
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metabolic engineering studies of Clostridium acetobutylicum strain M5 to produce butanol without acetone
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pta-deleted mutant, BK activity increases by 44%, ack-deleted mutant, similar activity as wild-type
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semi-automated reverse engineering algorithm. The reconstructed metabolic network was used to create a genome-scale model that correctly characterized the butyrate kinase knock-out and the asolventogenic M5 pSOL1 megaplasmid degenerate strains. Systematic gene knock-out simulations performed to identify a set of genes encoding clostridial enzymes essential for growth in silico.
H183L
the mutant shows 0.06% of wild type butyrate kinase II activity
H183Q
the mutant shows 0.19% of wild type butyrate kinase II activity
E334D
the mutant shows 0.29% of wild type butyrate kinase II activity
E332L
the mutant shows 0.34% of wild type butyrate kinase II activity
R215F E332D
the mutant shows 0.38% of wild type butyrate kinase II activity
E334Q
the mutant shows 0.41% of wild type butyrate kinase II activity
R215K
the mutant shows 0.44% of wild type butyrate kinase II activity
Results 1 - 10 of 17 > >>