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Results 1 - 10 of 10
EC Number
Amino acid exchange
Commentary
Reference
A273C
site-directed mutagenesis, the mutant is less thermostable than the wild-type enzyme
E336C
site-directed mutagenesis, the mutant is more thermostable than the wild-type enzyme, Tm is increased 3.7fold, half-life 1.8fold
E400I
site-directed mutagenesis, the mutant is more thermostable than the wild-type enzyme, Tm is increased 2.2fold, half-life 2.21fold
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construction of a pflB gene disruptant of strain W11 that barely utilizes glycerol under anaerobic conditions, but the growth of the DELTApfl mutant strain cultured with either glucose or dihydroxyacetone under anaerobic conditions is the same as that of wild-type strain W11
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construction of the pfl deletion resulting in two different strains LL1164 and LL1170 with two different phenotypes, high lactate and low lactate, respectively. Of eight colonies picked, two have the LL1164 phenotype and six have the LL1170 phenotype. Strain LL1170 consumes more cellobiose, produces more acetate and ethanol and less lactate than strain LL1164. A pfor/pfl double deletion strain LL1178 consumes about 70% of the 5 g/L cellobiose initially present, which is about the same as its parent strain LL1141. It requires sodium acetate for growth, even in the presence of yeast extract. Lactate is the main product of fermentation
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deletion of both pflA and pflB (strain RM220) partially restores the ability of the strain to respond to exogenous formate
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mutant strain CL3 with deletions in pflB, adhE, and frdA genes producing D-lactate from glucose, 44% slower growth under anaerobic conditions, 2.8fold higher glycolytic flux, 95% lactate yield, 22% lower ATP/ADP ratio
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mutant strain YK167 lacking pyruvate formate-lyase shows almost 50% increase in glucose consumption and flux with higher flux to lactate and lower ATP-yield, double mutant for pyruvate formate-lyase and lactate dehydrogenase (strain SE2382) shows 50% lower glucose flux and ATP yield than strain YK167 and results in reduced cell mass growth
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no formate production and decrease of unsaturation index in downstream (in absence of acetate) fatty acid production in mutants SPD0420M (enzyme mutant) and SPD1774M (activating enzyme mutant), restored by complementation, increased survival and lower disease sign score of mice infected with mutant strains, wild-type survival rate in complemented mutants, lower nasopharynx numbers and lower survival in lungs, intravenous infection with similar growth of mutant and wild-type. All mutant and wild-type strains grow better under aerobic than anaerobic conditions with glucose as sole carbon source, no difference in growth rate and yields between mutants and wild-type, with galactose (generally lower growth rate than with glucose) as carbon source all but strain SPD1774M grow better under aerobic conditions, strain SPD1774M better under anaerobic conditions, strain SPD0420M has lower growth rate than wild-type under anaerobiosis, the complemented strain SPD0420Comp grows better than SPD0420M in anaerobiosis. Mutants SPD035M and SPD0229M do not affect pyruvate formate lyase dependent product formation.
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recombinant coexpression of the enzyme with pyruvate format lyase activating enzyme and flavodoxin or ferredoxin in Saccharomyces cerevisiae leads to over 20fold increased expression of endogenous formate dehydrogenases FDH1 and FDH2
Results 1 - 10 of 10