EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.191 | N240A |
causes less than a 2fold reduction in specific activity, when assayed at substrate concentrations at 2fold above KM with the purified proteins |
696264 |
| 2.3.1.191 | N44A |
causes less than a 2fold reduction in specific activity, when assayed at substrate concentrations at 2fold above KM with the purified proteins |
696264 |
| 2.3.1.191 | Q32A |
causes less than a 2fold reduction in specific activity, when assayed at substrate concentrations at 2fold above KM with the purified proteins |
696264 |
| 2.3.1.191 | more |
construction of lpx insertional knockout mutations or RNAi knock-down mutants |
-, 720913 |
| 2.3.1.191 | more |
construction of lpxD1-null and lpxD2-null mutant strains, that show altered antibiotic susceptibility patterns, membrane permeability, but no innate immune responses |
-, 720944 |
| 2.3.1.191 | R293A |
KM((3R)-3-hydroxymyristoyl-[acyl-carrier protein]) increases 23fold compared to wild-type with little effect on kcat |
696264 |
| 2.3.1.191 | K46A |
mutation causes 3fold increase in KM((3R)-3-hydroxymyristoyl-[acyl-carrier protein]) and has no effect on kcat |
696264 |
| 2.3.1.191 | D232A |
mutation causes a 10fold reduction in kcat and a striking increase in the KM for both substrates |
696264 |
| 2.3.1.191 | N233A |
mutation causes a 10fold reduction in kcat and a striking increase in the KM for both substrates |
696264 |
| 2.3.1.191 | K194A |
mutation has little effect on activity |
696264 |
