EC Number |
Protein Variants |
Reference |
---|
2.3.1.167 | G38A |
activity against 10-deacetyltaxol is 1.45times more active than wild-type activity, activitys against 10-deacetylbaccatin III is slightly decreased |
757765 |
2.3.1.167 | F301A |
activity against 10-deacetyltaxol is 1.6times more active than wild-type activity, activitys against 10-deacetylbaccatin III is degraded |
757765 |
2.3.1.167 | F44A |
activity against 10-deacetyltaxol is nearly undetectable |
757765 |
2.3.1.167 | D390H |
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is 96% of the wild-type value |
757640 |
2.3.1.167 | I43S |
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold |
757640 |
2.3.1.167 | I43T |
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold |
757640 |
2.3.1.167 | D390R |
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.3fold |
757640 |
2.3.1.167 | I43S/D390R |
catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 3.3fold, compared to that of wild-type enzyme. Catalytic efficiency is 2.99fold higher than the catalytic efficiency of wild-type enzyme towards 10-deacetylbaccatin III and acetyl-CoA |
757640 |
2.3.1.167 | D166A |
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA |
755829 |
2.3.1.167 | D34E |
decrease of kcat/KM of 10-deacetylbaccatin III and acetyl CoA |
755829 |