EC Number |
Protein Variants |
Reference |
---|
2.3.1.135 | C161S |
hypomorphic mutant |
758452 |
2.3.1.135 | D128N |
site-directed mutagenesis, construction of an N-glycosylation site for enzyme membrane localization and orientation studies, overview |
674832 |
2.3.1.135 | E14L |
naturally occuring substitution localized in an N-terminal alpha-helix. The mutant protein is instable and shows accelerated proteosomal degradation. The instability of E14L does not abrogate the production of the visual chromophore in a cell-based assay. Eexpression of E14L leads to a rapid increase in cellular levels of retinoic acid upon retinoid supplementation |
756046 |
2.3.1.135 | I42N |
site-directed mutagenesis, construction of an N-glycosylation site for enzyme membrane localization and orientation studies, overview |
674832 |
2.3.1.135 | K104A |
site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme |
658075 |
2.3.1.135 | K133A |
site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme |
658075 |
2.3.1.135 | K133A/K134A |
site-directed mutagenesis, slightly increased activity compared to the truncated wild-type enzyme |
658075 |
2.3.1.135 | K134A |
site-directed mutagenesis, increased activity compared to the truncated wild-type enzyme |
658075 |
2.3.1.135 | K147A |
site-directed mutagenesis, reduced activity compared to the truncated wild-type enzyme |
658075 |
2.3.1.135 | K180A |
site-directed mutagenesis, nearly inactive mutant |
658075 |