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EC Number
Amino acid exchange
Commentary
Reference
D257G
random mutagenesis, the mutant of POMT-7, POMT7-M1, has a novel regioselectivity, from large scale mutant screening, mutant D257G methylates the 3-hydroxyl group of flavonols in addition to 7-hydrdoxyl group
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the recombinant SaOMT is used for 7-O-methylation of naringenin in recombinantly engineered production of 7-O-methyl aromadendrin via sakuranetin from 4-coumaric acid in Escherichia coli, overview. In order to increase the availability of malonyl-CoA, a critical precursor of 7-O-methyl aromadendrin, genes for the acyl-CoA carboxylase alpha- and beta-subunits (nfa9890 and nfa9940), biotin ligase (nfa9950), and acetyl-CoA synthetase (nfa3550) from Nocardia farcinica were also introduced
Results 1 - 2 of 2