EC Number   |
Protein Variants |
Reference |
|---|
    1.5.1.5 | more |
asparagine-125 is required for folate-substrate binding, arginine-173 mutation causes 500fold increase in the Km for NADP, serine-197 mutation a 20fold increase |
701402 |
| 1.5.1.5 | D121A |
kcat/Km for 5,10-methenyltetrahydrofolate is 600fold lower than wild-type value |
744335 |
| 1.5.1.5 | C58Y |
kcat/Km for 5,10-methenyltetrahydrofolate is 8.4fold lower than wild-type value |
744335 |
| 1.5.1.5 | G122D |
kcat/Km for 5,10-methenyltetrahydrofolate is 84fold lower than wild-type value |
744335 |
| 1.5.1.5 | K54S |
kcat/Km for 5,10-methenyltetrahydrofolate is identical to wild-type value |
744335 |
| 1.5.1.5 | more |
knockout mutant of MTHFD2 is embryonic lethal in mice at about 12 days gestation, no normal development of hematogenesis in the liver |
701402 |
| 1.5.1.5 | R653Q |
mutant enzyme has normal substrate affinity but a 36% reduction in half-life at 42°C |
698187 |
| 1.5.1.5 | D133E |
mutant retains no dehydrogenase activity |
701402 |
| 1.5.1.5 | more |
mutation of arginine-166 leads to complete loss of dehydrogenase activity, is critically responsible for binding of the inorganic phosphate, with arginine-198 mutation some dehydrogenase activity is retained, it probably only assists in phosphate binding |
701402 |
| 1.5.1.5 | D133E |
no dehydrogenase activity retained, Mg2+ binding site |
701402 |
