EC Number |
Protein Variants |
Reference |
---|
1.3.1.33 | C104A |
no detectable effect on the import of protein to plastid and processing in darkness |
676012 |
1.3.1.33 | C166A |
no detectable effect on the import of protein to plastid and processing in darkness |
676012 |
1.3.1.33 | C276A |
decrease in mature enzyme protein present in plastid and decrease in the amount of protochlorophyllide bound to enzyme. C276 constitutes the protochlorophyllide binding site in the active centre of enzyme |
676012 |
1.3.1.33 | C303A |
decrease in mature enzyme protein present in plastid and decrease in the amount of protochlorophyllide bound to enzyme. C303 constitutes a low affinity protochlorophyllide binding site involved in assembly and stabilization of imported enzyme inside etioplasts |
676012 |
1.3.1.33 | C103S |
the BchN subunit variant shows 0.5% residual activity and is essentially inactive |
687688 |
1.3.1.33 | C21S |
the BchN subunit variant shows 0.5% residual activity and is essentially inactive |
687688 |
1.3.1.33 | C46S |
the BchN subunit variant shows 0.5% residual activity and is essentially inactive |
687688 |
1.3.1.33 | Y189F |
mutant, the putative proton donor, Tyr 189, is replaced by a phenylalanine residue |
696150 |
1.3.1.33 | K197A |
mutant, constructed for analysing the role of the conserved active site lysine |
698913 |
1.3.1.33 | K197Q |
mutant, constructed for analysing the role of the conserved active site lysine |
698913 |