EC Number   |
Protein Variants |
Reference |
|---|
   1.14.14.3 | A74F |
site-directed mutagenesis, the mutant shows reduced activity and increased Km compared to the wild-type enzyme |
671985 |
| 1.14.14.3 | A74G |
site-directed mutagenesis, the mutant shows increased activity compared to the wild-type enzyme |
671985 |
| 1.14.14.3 | A75G |
site-directed mutagenesis, activity similar to the wild-type enzyme |
657939 |
| 1.14.14.3 | A75G/C106V/V173A |
site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum |
658060 |
| 1.14.14.3 | A75G/C106V/V173C |
site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum |
658060 |
| 1.14.14.3 | A75G/C106V/V173S |
site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum |
658060 |
| 1.14.14.3 | A75G/C106V/V173T |
site-directed mutagenesis, alpha-subunit residues, reduced activity compared to the wild-type enzyme, further red shift of emission spectrum |
658060 |
| 1.14.14.3 | A81H |
site-directed mutagenesis, residue of the alpha-subunit, mutant shows 13% of wild-type activity |
657927 |
| 1.14.14.3 | alphaDELTA262-290beta |
four times higher affinity for FMN than wild type |
348608 |
| 1.14.14.3 | alphaF114A |
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme due to reduced hydrophobicity of the active site |
671978 |
