EC Number |
Protein Variants |
Reference |
---|
1.14.13.83 | C358A |
no activity, no Fe-S center |
-, 698972 |
1.14.13.83 | C364A |
no activity, no Fe-S center |
-, 698972 |
1.14.13.83 | C394A |
no activity, no Fe-S center |
-, 698972 |
1.14.13.83 | C398A |
no activity, no Fe-S center |
698972 |
1.14.13.83 | C42A |
active, Fe-S center present |
-, 698972 |
1.14.13.83 | H390A |
active, Fe-S center present |
698972 |
1.14.13.83 | more |
for in vivo enzyme assays an Escherichia coli strain with all necessary genes for the production of cobyric acid except the cobG gene is used for complementation studies in combination with wild-type and mutant cobG genes (site-directed mutagenesis), for in vitro assays an Escherichia coli strain with a coupled multi-enzyme system (plasmid for the production of hydrogenobyrinic acid lacking the cobG gene) is combined with crude extract of an Escherichia coli strain overproducing GobG |
-, 698972 |