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Results 1 - 7 of 7
EC Number
Protein Variants
Commentary
Reference
C358A
no activity, no Fe-S center
C364A
no activity, no Fe-S center
C394A
no activity, no Fe-S center
C398A
no activity, no Fe-S center
C42A
active, Fe-S center present
H390A
active, Fe-S center present
more
for in vivo enzyme assays an Escherichia coli strain with all necessary genes for the production of cobyric acid except the cobG gene is used for complementation studies in combination with wild-type and mutant cobG genes (site-directed mutagenesis), for in vitro assays an Escherichia coli strain with a coupled multi-enzyme system (plasmid for the production of hydrogenobyrinic acid lacking the cobG gene) is combined with crude extract of an Escherichia coli strain overproducing GobG
Results 1 - 7 of 7