EC Number |
Protein Variants |
Reference |
---|
1.14.11.26 | A106T |
80% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate |
671443 |
1.14.11.26 | A177V |
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme |
-, 744105 |
1.14.11.26 | A311V |
random mutagenesis |
744105 |
1.14.11.26 | C155Y |
90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate |
671443 |
1.14.11.26 | C155Y/Y184H/V275I/C281Y |
580% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate |
671443 |
1.14.11.26 | C281Y |
200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate |
671443 |
1.14.11.26 | E16G/T90A/T304A |
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme |
744105 |
1.14.11.26 | E16G/T90A/T304A |
site-directed mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme |
744105 |
1.14.11.26 | E209Q |
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme |
744105 |
1.14.11.26 | E82D |
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme |
-, 744105 |