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Results 1 - 10 of 78 > >>
EC Number
Protein Variants
Commentary
Reference
A106T
80% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate
A177V
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme
A311V
random mutagenesis
C155Y
90% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate
C155Y/Y184H/V275I/C281Y
580% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate
C281Y
200% relative activity compared to the wild type enzyme using 1 mM penicillin G as substrate
E16G/T90A/T304A
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme; site-directed mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme
E209Q
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme
E82D
random mutagenesis, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme
F267L
random mutagenesis, active site mutation, the mutant shows increased activity with cephalosporin G compared to the wild-type enzyme
Results 1 - 10 of 78 > >>