EC Number   |
Protein Variants |
Reference  |
|---|
   1.13.11.50 | E69Q |
lower thermal stability of beta-sheet secondary structure, half catalytic center activity and remarkably silent difference in apparent substrate binding compared to the wild type enzyme |
675466 |
| 1.13.11.50 | H104E |
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions |
696107 |
| 1.13.11.50 | H104N |
approximately 1% of the specific activity of wild-type enzyme with substrate pentan-2,4-dione, retains binding affinity for Fe2+ at binding site I, binding seems to be tighter than in wild-type, small effect on other metal ions |
696107 |
| 1.13.11.50 | H62E |
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions |
696107 |
| 1.13.11.50 | H62N |
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, binding disruption of Cu2+, Mn2+, and Ni2+ compared with wild-type |
696107 |
| 1.13.11.50 | H64D |
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions |
696107 |
| 1.13.11.50 | H64E |
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions |
696107 |
| 1.13.11.50 | H64N |
conversion of substrate in a strictly Fe2+-concentration dependent manner, substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions |
696107 |
| 1.13.11.50 | more |
the exchange of 3 histidines in the Fe2+-binding centre shows that these histidines are crucial for for binding Fe2+ and for Fe2+-dependent dioxygenase activity |
696107 |
| 1.13.11.50 | E98A |
site-direted mutagenesis |
711267 |
