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Results 1 - 10 of 25 > >>
EC Number
Protein Variants
Commentary
Reference
E69Q
lower thermal stability of beta-sheet secondary structure, half catalytic center activity and remarkably silent difference in apparent substrate binding compared to the wild type enzyme
E98A
site-direted mutagenesis
E98Q
an interplay of residues Glu98, His104, Glu11 (from the neighbor subunit), and Arg80 is the most important for the Fe2+ transport in and out of the protein
E98Q
site-direted mutagenesis
F115A
site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme
F119A
site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme
F59A
site-directed mutagenesis, the mutant shows reduced turnover and altered iron binding compared to the wild-type enzyme
H104E
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions
H104N
approximately 1% of the specific activity of wild-type enzyme with substrate pentan-2,4-dione, retains binding affinity for Fe2+ at binding site I, binding seems to be tighter than in wild-type, small effect on other metal ions
H62E
no activity in initial rate assays with substrate pentan-2,4-dione, no binding of Fe2+ at binding site I, small effect on other metal ions
Results 1 - 10 of 25 > >>