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the purified enzyme HOX is immobilized on graphite electrodes. In the case of direct electronic wiring of HOX, O2 no longer acts as an electron acceptor, and it is the electrode that operates as an electron sink, which may affect the biocatalytic activity of enzymes due to the changed environment/conformation in the active site in the absence of a natural small molecular substrate. The formal potential of FAD in HOX estimated as a mean value of the anodic and cathodic peak potentials is less negative than that of free FAD adsorbed directly onto graphite electrodes
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