EC Number |
Protein Variants |
Reference |
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1.1.1.3 | more |
construction of a hom disruption mutant by insertional inactivation via double crossover leading to up to 4.3fold and 2fold increases in intracellular free L-lysine concentration and specific cephamycin C production, respectively, during stationary phase in chemically defined medium, overview |
-, 688167 |
1.1.1.3 | G378S |
construction of a homoserine dehydrogenase mutant HDG378S, encoded by hom1, in Corynebacterium glutamicum strain IWJ001, one of the best L-isoleucine producing strains. Strain HDG378S is partially resistant to L-threonine with the half maximal inhibitory concentration between 12 and 14 mM. Overexpression of lysC1, hom1 and thrB1 increased L-threonine and L-lysine production in Corynebacterium glutamicum ATCC13869 by 96folds and 21.2folds, respectively, overview |
-, 738802 |
1.1.1.3 | more |
construction of a hybrid enzyme AKIII-HDHI+ by fusing a wild-type monofunctional aspartate kinase AKIII enzyme to the thrA2+ gene, encoding the homoserine dehydrogenase including the interface region of the wild-type bifunctional enzyme, the hybrid enzyme shows highly improved kinetic properties for homoserine dehydrogenase activity, and is not sensitive to L-threonine inhibition |
654640 |
1.1.1.3 | more |
construction of transgenic Arabidopsis thaliana plants by transformation with gene akthr2 via Agrobacterium tumefaciens infection, determination of expression patterns of the gene akthr1 ans akthr2 in the transgenic plants |
657018 |
1.1.1.3 | H309A |
decrease of catalytic activity and elimination of substrate inhibition |
246402 |
1.1.1.3 | more |
design of an artificial allosteric enzyme to sense an unnatural signal for a precise and dynamical control of fluxes of growth-essential but byproduct pathways in metabolic engineering of industrial microorganisms. The natural threonine binding sites of the enzyme are engineered to a lysine binding pocket. The reengineered enzyme only responds to lysine inhibition but not to threonine |
739779 |
1.1.1.3 | more |
engineering of a Corynebacterium glutamicum strain HL1049 for effective production of methionine by elimination of the threonine synthesis gene and desensitizing the homoserine dehydrogenase versus inhibition by threonine, analysis of the amino acid spectrum of the engineered strain, overview |
688822 |
1.1.1.3 | G378E |
feedback resistance of the enzyme |
246394 |
1.1.1.3 | more |
generation of a knockout homoserine dehydrogenase (HSD) mutant by chemical mutagenesis. Auxotrophic mutant formed from ddh gene, encoding diaminopimelate dehydrogenase, recombinantly expressed in Corynebacterium glutamicum strain ATCC 13032 with blocked HSD shows increased yield of L-lysine of 24.89 g/l compared to ddh gene expressed in wild-type strain ATCC 13032 (20.66 g/l of L-lysine). The maximum yield of L-lysine for the auxotrophic mutant is attained at pH 7.5 and 30°C after 96 h incubation time. Method optimization, overview |
-, 762359 |
1.1.1.3 | more |
generation of HOM6-deleted (HOM6/hom6DELT and hom6DELTA/hom6DELTA) and HOM6-reintegrated (hom6DELTA/hom6DELTA::HOM6 and hom6DELTA::HOM6/hom6DELTA::HOM6) strains |
-, 740860 |