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Results 1 - 10 of 24 > >>
EC Number
Amino acid exchange
Commentary
Reference
G378E
feedback resistance of the enzyme
G378S
construction of a homoserine dehydrogenase mutant HDG378S, encoded by hom1, in Corynebacterium glutamicum strain IWJ001, one of the best L-isoleucine producing strains. Strain HDG378S is partially resistant to L-threonine with the half maximal inhibitory concentration between 12 and 14 mM. Overexpression of lysC1, hom1 and thrB1 increased L-threonine and L-lysine production in Corynebacterium glutamicum ATCC13869 by 96folds and 21.2folds, respectively, overview
H309A
decrease of catalytic activity and elimination of substrate inhibition
K105A
site-directed double-primer PCR mutagenesis
K105R
site-directed double-primer PCR mutagenesis
K205A
site-directed double-primer PCR mutagenesis
K57Aa
site-directed mutagenesis, in contrast to the wild-type enzyme, the mutant enzyme shows catalytic activity with NADP+, the activity with NAD+ is increased compared to the wild-type enzyme
L200F
site-directed mutagenesis, compared to mutant L200F, the double mutant shows 2 degree higher optimum temperature, 1.24 times higher activity, but the same pH optimum of pH 7.5 as mutant L200F. Both mutants L200F/D215K and L200F show good resistance to organic solvents and metal ions
L200F/D215A
site-directed mutagenesis
L200F/D215E
site-directed mutagenesis
Results 1 - 10 of 24 > >>