EC Number |
Protein Variants |
Reference |
---|
5.5.1.9 | E58V |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 91%, wild-type: 100% |
729167 |
5.5.1.9 | E60V |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 83%, wild-type: 100% |
729167 |
5.5.1.9 | F111L |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 88%, wild-type: 100%. Relative activity: 31% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type |
729167 |
5.5.1.9 | F116L |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 37%, wild-type: 100%. Relative activity: 4.7% (wild-type: 100%) |
729167 |
5.5.1.9 | F117L |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 89%, wild-type: 100%. Relative activity: 28% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type |
729167 |
5.5.1.9 | G108A |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 92%, wild-type: 100%. Relative activity: 90% (wild-type: 100%) |
729167 |
5.5.1.9 | G108E |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 0%, wild-type: 100%. Relative activity: below 0.5% (wild-type: 100%). Km (cycloeucalenol) increased compared to wild-type |
729167 |
5.5.1.9 | G108E |
in vivo conversion of cycloeucalenol into ergosterol and in vitro CPI activity in Saccharomyces cerevisiae erg7 transformants carrying Arabidopsis thaliana mutant CPI: 0% relative ergosterol synthesis (wild-type: 100%), below 0.5% in vitro microsomal CPI activity (wild-type: 100%) |
729167 |
5.5.1.9 | G108V |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 6%, wild-type: 100%. Relative activity: 1.7% (wild-type: 100%) |
729167 |
5.5.1.9 | G28E |
cycloeucalenol conversion into ergosterol in Saccharomyces cerevisiae erg7 transformants carrying mutagenized AtCPI, relative ergosterol synthesis: 15%, wild-type: 100%. Relative activity: 1% (wild-type: 100%) |
729167 |