EC Number |
Protein Variants |
Reference |
---|
5.3.2.6 | H6M |
the mutant has about 3fold increased specific activity compared with that of wild type enzyme |
748742 |
5.3.2.6 | H6M/A33E/F50V |
the mutation strongly enhances the activity for the Michael-type addition of butanal to trans-beta nitrostyrene, compared to the wild type enzyme |
748742 |
5.3.2.6 | M45Y/F50A |
the mutant enzyme displays low Michael-type addition activity compared to the wild type enzyme |
748742 |
5.3.2.6 | P1A |
inactive |
-, 749336 |
5.3.2.6 | P1A |
site-directed mutagenesis, inactive mutant |
718847 |
5.3.2.6 | P1A |
the mutation results in 430fold decreases in kcat/Km compared to the wild type enzyme |
726964 |
5.3.2.6 | R11A |
site-directed mutagenesis, inactive mutant |
718847 |
5.3.2.6 | R11A |
site-directed mutagenesis, no kinetic effects of the R11A mutation, the stereoselectivity of the R11A-catalyzed protonation at C-5 of the dicarboxylate substrate decreases, while the stereoselectivity of protonation at C-3 of the monocarboxylate substrate increases in comparison with wild-type 4-OT |
-, 718830 |
5.3.2.6 | R11A |
the mutation results in 280fold decreases in kcat/Km compared to the wild type enzyme |
726964 |
5.3.2.6 | R11A/R39A |
site-directed mutagenesis, inactive mutant |
-, 718830 |