EC Number |
Protein Variants |
Reference |
---|
4.3.1.2 | F170A |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 63% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
4.3.1.2 | T360S |
kcat (mesaconate) decreased compared to wild-type, Km (mesaconate) increased compared to wild-type. Conversion 2h: 76% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 86:14 (89:11 wild-type) |
729745 |
4.3.1.2 | L384A |
mutant displays pronounced amination activity towards 2-hexylfumarate ad decreased amination activity towards mesaconate compared to wild-type. Mutant displays a broad electrophile scope including fumarate derivatives with alkyl, aryl, alkoxy, aryloxy, alkylthio and arylthio substituents at the C2 position |
730442 |
4.3.1.2 | Q73A |
mutant displays significant decrease in ammonia addition and a large increase in methylamine addition compared to wild-type. Mutant displays a wide nucleophile scope including structurally diverse linear and cyclic allkylamines |
730442 |
4.3.1.2 | C361K |
mutant shows no reactivity. Conversion 2h: 10% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
4.3.1.2 | Q172N |
mutant shows no reactivity. Conversion 2h: 6% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |
4.3.1.2 | Q73N |
mutant shows very low-level amination activity preventing the measurement of kinetic parameters. Conversion 2h: 18% (73% wild-type), diastereomeric product ratio (threo:erythro) after 2h: 95:5 (89:11 wild-type) |
729745 |