EC Number   |
Protein Variants |
Reference |
|---|
   4.1.3.43 | L87A |
mutant generated by site-directed mutagenesis, variant exhibits a 40fold preference for propionaldehyde over acetaldehyde in contrast to wild type with similar specificities for acetaldehyde and propionaldehyde |
714244 |
| 4.1.3.43 | L87N |
mutant created by site-specific mutagenesis |
722458 |
| 4.1.3.43 | L87N/Y290F |
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control |
722458 |
| 4.1.3.43 | L87W |
mutant created by site-specific mutagenesis |
722458 |
| 4.1.3.43 | L87W/Y290F |
exhibits stereoselectivity opposite to that of the wild type and alkyl chain length of the aldehyde does not affect stereochemical control |
722458 |
| 4.1.3.43 | L89A |
mutant generated by site-directed mutagenesis, specificity constant of this variant in the aldol addition reaction using pentaldehyde is increased approx. 50fold, making it more catalytically efficient for pentaldehyde utilization compared to the wild-type utilization of the natural substrate, acetaldehyde |
714244 |
| 4.1.3.43 | L89A |
reduction in channeling efficiency of 30% for all aldehydes tested |
721651 |
| 4.1.3.43 | more |
all mutations created by site specific mutagenesis |
721651 |
| 4.1.3.43 | more |
mutants created by site-specific mutagenesis |
722458 |
| 4.1.3.43 | R16A |
mutant generated by site-directed mutagenesis, no detectable aldol cleavage and pyruvate R-proton exchange, supporting the role of Arg-16 in stabilizing a pyruvate enolate intermediate |
714244 |
