EC Number |
Protein Variants |
Reference |
---|
4.1.1.18 | T88N |
site-directed mutagenesis, the mutant is expressed in inclusion bodies and shows no clear activity |
747396 |
4.1.1.18 | T88P |
site-directed mutagenesis, the mutant is expressed in inclusion bodies and shows no clear activity |
747396 |
4.1.1.18 | T88Q |
site-directed mutagenesis, the mutant is expressed in inclusion bodies and shows no clear activity |
747396 |
4.1.1.18 | T88S |
site-directed mutagenesis, the mutant shows higher thermostability with a 2.9fold increase in the half-life at 70°C (from 11 min to 32 min) and increased melting temperature (from 76°C to 78°C). The specific activity and pH stability of T88S at pH 8.0 are increased to 164 U/mg and 78% compared to 58 U/mg and 57% for the wild-type enzyme. The productivity of cadaverine with T88S is 40 g/l/h in contrast to 28 g/l/h with wild-type enzyme. The mutant is a promising biocatalyst for industrial production of cadaverine. No additional hydrogen bond is formed when T88 is substituted by D, F, or S, and the improved stability may be attributed to the favorable atom and torsion angle potentials |
747396 |
4.1.1.18 | V147F |
site-directed mutagenesis, the mutant shows increased LDC activity |
-, 747358 |
4.1.1.18 | V147F/E583G |
site-directed mutagenesis, the mutant shows 1.62fold increased LDC activity compared to wild-type enzyme |
-, 747358 |
4.1.1.18 | V91C/G445C |
site-directed mutagenesis, mutant A1 |
-, 747369 |