EC Number |
Protein Variants |
Reference |
---|
4.1.1.18 | A52C/P54D/T55S |
the ratio of activity with L-Orn to activity with L-Lys is 2.7, compared to 0.69 for the wild-type enzyme |
654984 |
4.1.1.18 | E583G |
site-directed mutagenesis, the mutant shows 1.32fold increased LDC activity and 1.48fold improved productivity of cadaverine compared to wild-type enzyme |
-, 747358 |
4.1.1.18 | F102C/T544C |
site-directed mutagenesis, mutant A2 |
747369 |
4.1.1.18 | F14C/K44C |
site-directed mutagenesis, mutant B1, the disulfide bond mutation in the decameric interface of wild-type CadA improves its structural stability, and as a result, enhances the pH and thermal stabilities along with organic solvent tolerance, but reduces the catalytic efficiency, compared to the wild-type |
-, 747369 |
4.1.1.18 | F14C/K44C/L7M/N8G |
site-directed mutagenesis, the disulfide bond mutation in the decameric interface of wild-type CadA improves its structural stability, and as a result, enhances the pH and thermal stabilities along with organic solvent tolerance compared to the wild-type, addition of mutations L7M and N8G to mutant B1 slightly increases the catalytic efficiency compared to mutant B1 but remains still lower than wild-type |
-, 747369 |
4.1.1.18 | G319W |
the ratio of turnover number to Km-value obtained with L-Orn relative to that obtained with L-Lys as substrate is 3.9, compared to 0.83 for the wild-type enzyme |
655829 |
4.1.1.18 | K2C/G227C |
site-directed mutagenesis, the mutant shows reduced affinity for pyridoxal 5'-phosphate and reduced activity compared to wild-type |
749101 |
4.1.1.18 | L89R |
the mutant elutes at the expected position for an LdcI dimer (about 150000 Da), the mutant shows about 5fold lower level of activity than wild type and this activity is not inhibited by ppGpp |
714832 |
4.1.1.18 | M50V |
the ratio of activity with L-Orn to activity with L-Lys is 0.64, compared to 0.69 for the wild-type enzyme |
654984 |
4.1.1.18 | M50V/A52C |
the ratio of activity with L-Orn to activity with L-Lys is 1.9, compared to 0.69 for the wild-type enzyme |
654984 |