EC Number   |
Protein Variants |
Reference |
|---|
   3.6.5.4 | A254L |
no growth defect |
-, 658538 |
| 3.6.5.4 | A334W |
mutant, exhibits no significant GTP hydrolysis |
687902 |
| 3.6.5.4 | A335W |
mutant, GTPase activation defective |
687905 |
| 3.6.5.4 | A336W |
mutant, GTPase activation defective |
687905 |
| 3.6.5.4 | C86A |
mutations at C86 yield a more complex pattern: whereas C86A and C86U completely abolish GTPase activation by the RNA, C86 and C86G reduce GTPase activity by only 50% |
734245 |
| 3.6.5.4 | C86G |
mutations at C86 yield a more complex pattern: whereas C86A and C86U completely abolish GTPase activation by the RNA, DELTAC86 and C86G reduce GTPase activity by only 50%. Despite defective GTP hydrolysis, the G83A mutant shows any detectable defect in the efficiency of GTPase docking at the distal end |
734245 |
| 3.6.5.4 | C86U |
mutations at C86 yield a more complex pattern: whereas C86A and C86U completely abolish GTPase activation by the RNA, C86 and C86G reduce GTPase activity by only 50% |
734245 |
| 3.6.5.4 | C87A/C97U |
site-directed mutagenesis, combining C97U with C87A generates a superactive SRP RNA double mutant that hydrolyzes GTP 5.5fold faster than wild-type SRP RNA |
734245 |
| 3.6.5.4 | C97U |
site-directed mutagenesis, the mutant prolongs GTPase docking at the distal end, which correlates with its faster GTP hydrolysis rate |
734245 |
| 3.6.5.4 | C97U/G99A |
site-directed mutagenesis, combining G99A with C87A generate s a superactive SRP RNA double mutant that hydrolyzes GTP 4.6fold faster than wild-type SRP RNA |
734245 |
